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#26 2009-09-22 01:08:03

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

Time to get out and about collecting fungi from which to rear interesting Diptera.  Flies in the Platypezidae are found in fungi and are distinctive by their flattened rear tarsal segments.  Even in this dry weather it is possible to find interesting fungi on logs or damp areas.  The first two photos show a fungus called the Bay Polypore, Polyporus badius (P durus) which has a chestnut to red-brown shiny cap and a whitish layer of small pores underneath.  The second photograph shows that the pore layer has some larval tunnels.  This cap was set up to rear over moist coir fibre and the first fly to emerge (a male)  is shown alive and then pinned.  The males are jet black when fully hardened off, but the females are a dull brown.  These are not very good photos, but this fly is identifiable using Peter Chandler's key to the family Platypezidae as Seri obscuripennis.  This is a very uncommon fly (uncommon by normal catching methods i.e. sweeping) which used to have Red Data book status.  However since I have been rearing Diptera from fungi, I have found it commonly in Oxfordshire by rearing from this specific Bay Polypore fungus.  It seems to be its only food source. 

I hope this might interest others to try rearing if you find this fungus.  I have reared flies from it even if no tunnels are visible.  More records of Seri obscuripennis are needed in order to understand its distribution and how rare (or not) it really is.   I would be delighted to hear from anybody who finds it.

Judy


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#27 2010-02-17 17:34:15

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

Rearing time coming up again!  The end of Feb down south is a really good time to collect dead wood or moss mat or swamp litter for rearing diptera.  In northern climes, one would do better to wait until the end of March to collect material, but I shall be out looking this next weekend.  Another good source is rotting old bracket fungi - for example the birch polypore, Piptoporus betulinus, brackets that have fallen off the tree and become half rotted (usually very slimy) around the base of the tree, or brackets of the turkey tail, Trametes versicolor.  Don't forget waterlogged dead wood from log jams in small streams.  Here are some larvae collected from rotting Turkey tail brackets a few weeks ago.  No idea what they are but I'm waiting with interest!

Any ideas, anyone?
Judy


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#28 2010-02-17 18:33:06

muscapaul
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Name: Paul Beuk
From: Maastricht, Netherlands
Registered: 2010-01-06
Posts: 349
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Re: Rearing Diptera

What type of coir fibre do you use and how do you obtain it?
Do you humidify it once and then leave it be or do you ad moisture every now and then?
Can you indicate 'how humid' it should be?

PS: I would not mind a good instruction in this forum: http://www.diptera.info/forum/viewforum.php?forum_id=9. wink

Last edited by muscapaul (2010-02-17 18:36:42)

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#29 2010-03-25 21:30:47

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

Sorry, occasionally work gets too busy for fun with flies. Here are some photos of the method for rearing that I use.  For rearing from fungi I use coir fibre blocks or briquettes.  These are available to buy on the internet, but usually in rather too large quantities for convenience.  I have a friend who buys them in bulk for making his own compost and I buy them from him as I need them.  They need to be re-hydrated by soaking in a bucket with some water (needs to be damp, but not soaking). This makes rather a lot of material.  If I only want a small quantity, I hacksaw a chunk off one end of a block and re-hydrate that.  One can buy bags of loose coir fibre from garden centres, already hydrated.  One could use non-soil potting compost, but of course one does not need all the plant food that is added, the only thing the fly larvae need is a suitably moist medium of the correct structure for pupation.  I put the re-hydrated coir fibre into a plastic pot to about one third depth.  Supermarket food pots as shown are free and ideal in size.  After placing the fungal cap or bracket on the moist fibre, a white ex-curtain net or horticultural fleece portion can be secured over the pot with an elastic band.  Round pots are better - square pots are not so easy to make secure so the flies don’t make an escape under the elastic band.  The pots need to be checked weekly and sprayed with water if they are getting dry.

I keep the pots indoors, so they are warm. Under these conditions, fungus gnats can be emerging within two weeks.  Some flies take much longer.  If you put the pots outside in a shed, obviously they will be cooler and everything takes much longer to emerge.  They need to be checked daily after about a week to avoid missing things.  Inserting the end of the pooter under the net and catching the emerging flies is tricky and often they escape and go to the nearest window, where you can have even more fun chasing them with your pooter.  Some Platypezid larva collected in fungi in the autumn will not emerge as adults until the next year, so you need to be patient.  Some larvae need to have a cooler/cold period to mimic winter and then be brought into the warm to encourage emergence.  Teneral flies need to be allowed to harden up for a day at least before pinning, or colours will not be correct and their bodies may collapse.

For very small toadstools, rearing can be achieved on a miniature scale in a small plastic tube with coir fibre.  In the photo of the small tube one can see white mycetophilid fungus gnat pupae in cocoons at the junction with the coir. All records are most valuable if the fungus collected is properly identified to species in the first case.  I recommend going out with your local fungus group and asking for spare, named fungal specimens.  Even if the caps look look pristine and with no larvae, fly eggs are very often present and larvae appear in a short time.  Some fungi start emerging in the spring, so one can start in a month or so!
Judy


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Last edited by Judy Webb (2010-03-25 21:45:40)

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#30 2010-03-26 22:35:50

muscapaul
Registered user
Name: Paul Beuk
From: Maastricht, Netherlands
Registered: 2010-01-06
Posts: 349
Website

Re: Rearing Diptera

Thanks.

If you ever find time, you are still kindly invited to leave this information on Diptera.info as well, perhaps as a short article.

Paul

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#31 2010-05-25 02:05:29

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

If you go out now and find some brackets of the Turkey tail, Trametes versicolor, on logs in a damp or wet place, you might find some Platypezid puparia just under the surface of the gill layer, as in these photos, ready to hatch.  in these photos I just scratched off the frass lumps and found circular holes with a puparium visible in each.  They are set up to rear and watch this space to see if I am lucky with flies soon...
Judy


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#32 2010-05-25 03:16:11

muscapaul
Registered user
Name: Paul Beuk
From: Maastricht, Netherlands
Registered: 2010-01-06
Posts: 349
Website

Re: Rearing Diptera

Tempting to go out, but I think the wife will not be happy with me going collecting with the imminent move to the Maastricht area and too many things to arrange. Hope I will have better a chance next year...

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#33 2010-05-27 00:08:11

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

Not long to wait, because here are the flies!  All black males emerged first, then one really beautiful silver and black female. No new discoveries here, from the female pattering this appears to be the Platypezid Polypivora ornata which is well known to breed in Trametes versicolor.  Photography was very difficult because, although the flies were reluctant to fly, they were constantly rushing about randomly all over the surface.  Persuaded them to walk onto my finger, but mostly too active until they ran up my arm onto my black fleece.  Then both sexes suddenly became still and sat motionless for some of the photos attached here to be taken.  The silver patches on the female flashed in the sunlight as she moved.

Judy


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Last edited by Judy Webb (2010-05-27 00:10:50)

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#34 2010-09-25 01:00:38

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

All this rain since August has meant that this looks like being the best year for fungal fruiting for 10, maybe 20 years!  I've already got lots of little pots with caps on the go rearing Diptera.  I encourage anybody out there with fungi emerging nearby to collect some caps and look for larvae.  If you don't know what the fungi are, take some photos of them before setting up to to rear as in this thread.  Then if you get any good flies or fungus gnats, post the photo of the fungus here and I will have  a go at identifying it, so that you can tie the fly to a specific food source.

Here are some nice little fungus gnats newly emerged from white Postia (Tyromyces) bracket fungi on dead wood. This species seems specific to Postia.

Judy


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#35 2011-01-06 20:59:55

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

Latest rearing !! - yesterday from a pot set up with  a cap of Pluteus cervinus from a woodland near Oxford, collected in October last year.  Some pretty female Platypezids.  A couple of all black males emerged from the same pot this evening.

Some poor photos, but anybody any ideas on what species?

A reminder that Platypezids are one of the featured groups of the DF Advanced Workshops this March at Preston Montford.  No doubt I'll be able to identify them after that course!

Judy


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#36 2011-01-12 22:49:10

Judy Webb
Committee
Name: Judith Webb
Registered: 2008-02-21
Posts: 375

Re: Rearing Diptera

Peter Chandler has replied in the Identification Forum to my other post.  He thinks this platypezid is Paraplatypeza bicincta, which is a very scarce thing.  A few recent records in southern counties and this is the first time it has been reared (!) so that its food source can be now positively identified as Pluteus cervinus, a reasonably common species of toadstool found on dead wood.  The commoner species developing in Pluteus cervinus is Paraplatypeza atra, which I have reared from this toadstool before (all black females and males).

A good reason to target collecting more of this toadstool next autumn!
Judy

Last edited by Judy Webb (2011-01-19 15:03:37)

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